Genome-wide association mapping of quantitative traits in NMRI outbred mice
Our genome wide assoication study using 288 outbred NMRI mice was performed to map loci influencing HDL cholesterol, systolic blood pressure, triglyceride levels, glucose, and urinary albumin-to-creatinine ratios. Tail-cuff blood pressure was measured in 8-week-old mice, and systolic blood pressure (SBP), diastolic blood pressure (DPB) and mean arterial pressure (MAP) was calculated. Once the mice were 10-weeks-old, urine samples were collected, and urinary albumin and creatinine concentrations were measured. A series of mouse albumin standards was also quantified. Blood samples were obtained from each mouse and measurement of total cholesterol (CHL), HDL cholesterol (HDL), triglycerides (TG), and glucose (GLU) performed. Genomic DNA samples were genotyped using the Mouse Diversity Genotyping Array (Yang et al. 2009). For further details see Zhang et al. 2011.
Genome-Wide Association Mapping of Quantitative Traits in Outbred Mice