Project F: Genetics of mRNA processing
Joel H. Graber (Jackson)
The molecular response to environmental changes, such as diet, can involve changes in gene expression at any stage from DNA through RNA to final protein product. The genetic bases for differences in post-transcriptional processing, such as alternative splicing and alternative polyadenylation, remain poorly understood. Genetically defined strains of mice, such as the Diversity Outbred and recombinant inbred lines (RIX), will provide a powerful means of investigating genetic differences in gene expression and regulation in response to controlled changes in environment. We will work in collaboration with Projects C, D, and E to investigate and characterize genetic control of mRNA processing in response to dietary variation, focusing on both trans-acting factors and the local sequence elements that together control alternative processing.